Emma is the daughter of industrialist John Night, and the goddaughter of Hugo Drummond. Her father however was murdered because his industry was competing against the United States over contract rights in supplying a United Nations intelligence department. She is a feminist heroine, a master of martial arts and a formidable fencer. A certified genius, she specializes in chemistry and other sciences. Her husband, Peter Peel, was a pilot whose plane disappeared over the Amazonian forest.
In 1958 she was teamed up with Drummond and Jimmy Bond to track down Allan Quatermain and Mina Murray, who had stolen the Black Dossier from MI5. The chase took them from Londen to Scotland, where the two escaped with help of the Galley-Wag, but not before revealing to Drummond that it was Jimmy who had killed John Night. Jimmy killed Drummond as well to prevent him from telling this to Emma, and told Emma Allan and Mina had killed him, offering consolation for her.
In 2009, now an old woman, Emma Night had become the new M. By this time she had learned the truth about her father's and godfather's death from disenchanted CIA operative Michael Westin. This motivated her to re-evaluate the League and discover that they were in fact the heroes. She was contacted by Orlando who needed to find Mina, who had been missing for 40 years. In return for the location of the Pool of Fire that grants all who bathe in it immortality, she had Mina tracked down in a lunatic asylum. She also told Orlando she was keeping the aging Bond alive as long as possible, despite the fact that he was in great agony from several diseases.
After the battle with the Antichrist was over, Emma resigned from MI5 and, along with two other resigned agents, she helped move the body of Allan Quatermain, who had been killed during the battle, to Africa to be buried. She was last seen departing for locations unknown along with Mina and Orlando.
Emma Night is a thinly-veiled version of Emma Peel (maiden name Emma Knight) from the British television series The Avengers. The old Emma seen in 2009 greatly resembles Judi Dench's M from the James Bond films.